分泌表达pEGF的重组酿酒酵母在断奶仔猪上的应用研究

doi:10.11843/j.issn.0366-6964.2016.05.011

Abstract

Abstract:

The application research of recombinant pEGF-expressed S.cerevisiae in early-weaned piglets was verified in the present research.A total of 72 piglets (body weight was (6.10±0.15)kg) weaned at 21-24 day of age were randomly assigned to 3 groups (4 replicates in each group，6 per replicate)：control group (basal diet＋228 mL•kg^-1 culture medium)，empty vector-expressed S.cerevisiae group (basal diet＋228 mL•kg^-1 INVSc1(EV))，and recombinant EGF-expressed S.cerevisiae group (basal diet＋228 mL•kg^-1 INVSc1(pYES2-Mfα-spEGF)).At day 21，one piglet from each pen were randomly chosen to be killed to investigate the morphology，the levels of IgA，IgG and IgM，the enzyme activities and mRNA expression levels of ALP，CK and LDH，and the mRNA expression of EGF-R in the intestinal mucosa.As shown in this study，the mRNA expression levels of digestive enzymes (ALP，CK and LDH) and EGF-R (P<0.05)，the activities of CK and LDH (P<0.01)，the levels of IgA，IgG and IgM (P<0.05)，the ADG (P<0.05) were significantly increased，and F/G(P<0.05) was significantly decreased in the recombinant pEGF-expressed S.cerevisiae group compared with the control and INVSc1(EV) groups.Herein，recombinant pEGF-expressed S.cerevisiae in this study have higher biological activities，including improving intestinal development and immune function，which will be applied in the swine industry.

CLC Number:

S828
S816.32

WANG Shu-jin，WANG Bin-xing，GUO Chun-hua，BAI Xue，PENG Zhong-li，ZHANG Zheng-fan，ZHOU Lin. Application Research of Recombinant EGF-expressed Saccharomyces cerevisiae in Early-weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 944-954.

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Application Research of Recombinant EGF-expressed Saccharomyces cerevisiae in Early-weaned Piglets

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